The Next Generation of Therapies

unimportant decoration Nano-scale droplet illustration

NanoBio® Protect

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BlueWillow’s  proprietary NanoBio® technology platform uses nano-scale droplets to enable numerous topical antiseptic products. NanoBio® products persist on skin for 4+ hours and hydrate the skin to help avoid cracking where germs can enter. The products are comprised of droplets 300-600nm in size that have a common over-the-counter antiseptic agent placed on their surface. As shown at right, the nano-droplets physically disrupt the outer membrane of germs, killing on contact.


NanoBio Protect logo

BlueWillow’s NanoBio® Protect Nasal Antiseptic Swabs are alcohol-free, and can be used on skin inside the nose very similar to how hand sanitizers are used to clean a person’s hands. The product has been shown in lab tests to kill 99.99% of germs and offers several unique advantages:

  • NanoBio® Protect is alcohol-free and non-irritating when applied to skin
  • The product is comprised of soft, biodegradable nanodroplets that persist on skin for 4+ hours
  • The droplets are formulated with a common OTC skin antiseptic, which is placed on the surface of the droplets to make it readily available to kill germs
  • NanoBio® Protect hydrates skin following use, is fragrance-free and leaves no residue
  • The product is stable at room temperature for 3 years
  • Manufacturing of NanoBio® Protect has been scaled to commercial levels
  • The product has patent coverage through 2029 (issued) and 2039 (pending)

When tested in standard in vitro lab models, NanoBio® Protect completely killed bacteria and enveloped viruses tested within 60 seconds of exposure, as shown below. BlueWillow has not completed clinical studies to confirm similar results in humans.


As shown below, ex vivo tests in human skin demonstrate that NanoBio® Protect persists on skin up to 7x better than commercial products or aqueous solutions containing the same antiseptic agent:

Studies in human volunteers demonstrate that a single application of NanoBio® Protect significantly increases skin hydration for at least 3 hours, as compared to common hand sanitizer products:

Finally, published research demonstrated that three NanoBio nasal antiseptic formulations significantly enhanced survival in mice that were subsequently challenged with a lethal dose of influenza virus 90 minutes post-treatment (p<0.005):


Research and Scientific Publications

Scientific publications describing the extensive research conducted using topical NanoBio-based applications are listed below:

  • “A Nanoemulsion as an Effective Treatment Against Human Pathogenic Fungi”. Therapeutics and Prevention. 2019, Nov/Dec 4:6
  • Screening of Nanoemulsion Formulations and Identification of NB-201 as an Effective Topical Antimicrobial for Staphylococcus aureus in a Mouse Model of Infected Wounds”. Military Medicine. 2016, May: 181, 5S:259-264
  • “Nanoemulsion Therapy for Burn Wounds Is Effective as a Topical Antimicrobial Against Gram-Negative and Gram-Positive Bacteria”. Burn Care Res. 2016, March /April: 37(2); 104-114
  • “Treatment With a Novel Topical Nanoemulsion (NB-001) Speeds Time to Healing of Recurrent Cold Sores”. Drugs Dermatol. 2012 Aug; 11(8):970-7
  • “In Vitro Antibacterial Activity of NB-003 against Propionibacterium acnes”. Antimicrobial Agents And Chemotherapy, Sept. 2011,Vol. 55, No. 9, p. 4211–4217
  • “Topical Nanoemulsion Therapy Reduces Bacterial Wound Infection And Inflammation After Burn Injury”. Surgery. 2010
  • “NB-002, A Novel Nanoemulsion With Broad Antifungal Activity Against Dermatophytes, Other Filamentous Fungi, And Candida Albicans”. Antimicrobial Agents And Chemotherapy, Aug. 2009, Vol. 53, No. 8, p. 3273–3279
  • “In Vitro Activities of a Novel Nanoemulsion against Burkholderia and Other Multidrug-Resistant Cystic Fibrosis-Associated Bacterial Species”. Antimicrobial Agents And Chemotherapy, Jan. 2009, Vol. 53, No. 1, p. 249–255
  • “The Fungicidal Activity Of Novel Nanoemulsion (X8W60PC) Against Clinically Important Yeast And Filamentous Fungi”. Mycopathologia 155: 195–201, 2001
  • “A Novel Surfactant Nanoemulsion With A Unique Non-Irritant Topical Antimicrobial Activity Against Bacteria, Enveloped Viruses And Fungi”. Microbiol. Res. (200I) 156, I-7
  • “Inactivation Of Ebola Virus With A Surfactant Nanoemulsion”. Acta Tropica 87 (2003) 315/320
  • “Antimicrobial Mechanism Of Action Of Surfactant Lipid Preparations In Enteric Gram-Negative Bacilli”. Journal of Applied Microbiology 2000, 89, 397-403
  • “Prevention of Murine Influenza A Virus Pneumonitis by Surfactant Nano-emulsions”. Antiviral Chemistry & Chemotherapy, 2000, 11:41-49
  • “A Novel Surfactant Nanoemulsion with Broad-Spectrum Sporicidal Activity Against Bacillus Species”.  The Journal of Infectious Diseases, 1999, 180:1939–49